耐甲氧西林金黄色葡萄球菌诱导牛乳腺上皮细胞炎症反应的分子标志物研究

doi:10.11843/j.issn.0366-6964.2016.10.007

Abstract

Abstract:

The aim of this study was to acquire key molecular marker related to inflammatory reaction of bovine mammary epithelium cells induced by Methicillin-resistant Staphylococcus aureus (MRSA).In this study，bovine mammary epithelium cell line (Mac-T) was infected with MRSA in vitro by compared with the cells treated by S.aureus as well the untreated cells.The expression levels of two candidate genes associated with subclinical mastitis ((trafficking protein particle complex 9(TRAPPC9)，Janus Kinase 2(JAK2)) and one pro-inflammatory gene (interleukin 6，IL-6) were detected and compared among infected groups and non-infected group at different time points.Comparing with the untreated cells，the results showed that the mRNA expression level of TRAPPC9 significantly decreased (P<0.05) in the Mac-T cells at 6 h post stimulation by MRSA，while that of JAK2 significantly decreased (P<0.05) at 8 h；And the expression levels of TRAPPC9 and JAK2 in the cells significantly decreased (P<0.05) at 6 h post infection by S.aureus.Compared with S.aureus stimuli，the expression levels of TRAPPC9，JAK2 and IL-6 in the Mac-T cells were moderately higher at 6 and 10 h post infection by MRSA.Especially，the expression level of TRAPPC9 was significantly higher in MRSA treated cells (P<0.05) compared to S.aureus treated cells at 6 h post infection.These results suggest that the genes expression changes in the Mac-T cells were similar after the infection of MRSA and S.aureus，while the gene expression changes of JAK2 in the cells was delayed from MRSA infection compared to S.aureus.TRAPPC9 can be used as the preferred molecular marker for detecting MRSA and S.aureus infection in bovine mammary epithelium cells.

CLC Number:

S823
S813.3

SONG Min-yan，WEI Yi-yuan，Muhammad Zahoor Khan，WANG Xin，YU Ying. Molecular Marker Study of Inflammatory Reaction in Bovine Mammary Epithelium Cell Line Induced by Methicillin-Resistant Staphylococcus aureus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 1995-2002.

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Molecular Marker Study of Inflammatory Reaction in Bovine Mammary Epithelium Cell Line Induced by Methicillin-Resistant Staphylococcus aureus

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